(A) Freshly prepared MDMs from healthy donors (n = 4) cultured in the presence or absence of γ-M. tuberculosis (10 μg/mL). Some γ-M. tuberculosis–cultured wells supplemented with metabolites were enriched in the plasma of nonconverters (4-hydroxypyridine, dl-methionine sulfoxide, l-kynurenine, l-α-glycerophosphocholine, d-sedoheptulose 7-phosphate, deoxycorticosterone acetate). After 72 hours, the expression (MFI) of Siglec-14 was determined by flow cytometry. (B) MDMs from healthy donors (n = 8) were infected at an MOI of 2.5. Some of the infected MDMs were cultured in the presence of the metabolites 4-hydroxypyridine, dl-methionine sulfoxide, l-kynurenine, l-α-glycerophosphocholine, d-sedoheptulose 7-phosphate, and deoxycorticosterone acetate (each 100 μM). Intracellular CFUs were determined at 5 days postinfection. P values were determined by unpaired 2-tailed t test. The mean ± SD is shown. (C) In the above panel, the supernatant was aspirated, and the level of HBD2 and S100A12 was determined by ELISA. P values were determined by unpaired 2-tailed t test. The mean ± SD is shown. (D) MDMs from healthy donors (n = 5) were isolated and transfected with siRNA targeting Siglec-14 or control siRNA and infected with M. tuberculosis H37Rv at an MOI of 2.5. In some M. tuberculosis–infected wells, deoxycorticosterone acetate (100 μM) was added. After 5 days, CFUs were counted. P values were determined by 1-way ANOVA with Tukey’s multiple comparisons test. Data are representative of 4 independent experiments. The mean ± SD is shown. (E) The concentrations of HBD2 and S100A12 were determined by ELISA. The P values were determined by unpaired t test. The mean ± SD is shown.