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Usage Information

Leveraging copper import by yersiniabactin siderophore system for targeted PET imaging of bacteria
Nabil A. Siddiqui, … , Suzanne E. Lapi, Nalinikanth Kotagiri
Nabil A. Siddiqui, … , Suzanne E. Lapi, Nalinikanth Kotagiri
Published May 24, 2021
Citation Information: JCI Insight. 2021;6(10):e144880. https://doi.org/10.1172/jci.insight.144880.
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Resource and Technical Advance Infectious disease

Leveraging copper import by yersiniabactin siderophore system for targeted PET imaging of bacteria

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Abstract

There is an emerging need for accurate and rapid identification of bacteria in the human body to achieve diverse biomedical objectives. Copper homeostasis is vital for the survival of bacterial species owing to the roles of the metal as a nutrient, respiratory enzyme cofactor, and a toxin. Here, we report the development of a copper-64–labeled bacterial metal chelator, yersiniabactin, to exploit a highly conserved metal acquisition pathway for noninvasive and selective imaging of bacteria. Compared with traditional techniques used to manufacture probes, our strategy simplifies the process considerably by combining the function of metal attachment and cell recognition to the same molecule. We demonstrate, for the first time to our knowledge, how a copper-64 PET probe can be used to identify specific bacterial populations, monitor antibiotic treatment outcomes, and track bacteria in diverse niches in vivo.

Authors

Nabil A. Siddiqui, Hailey A. Houson, Nitin S. Kamble, Jose R. Blanco, Robert E. O’Donnell, Daniel J. Hassett, Suzanne E. Lapi, Nalinikanth Kotagiri

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Usage data is cumulative from December 2022 through December 2023.

Usage JCI PMC
Text version 456 141
PDF 77 36
Figure 99 0
Table 29 0
Supplemental data 18 1
Citation downloads 25 0
Totals 704 178
Total Views 882

Usage information is collected from two different sources: this site (JCI) and Pubmed Central (PMC). JCI information (compiled daily) shows human readership based on methods we employ to screen out robotic usage. PMC information (aggregated monthly) is also similarly screened of robotic usage.

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