(A) Fundus imaging in a primate eye after injection of 5 × 10¹⁰ particles of NHP#26-scCAG-GFP revealed a disc of GFP expression centered on the fovea and a punctate pattern of GFP expression across the retina. (B) Confocal imaging of native GFP expression in the flat-mounted fovea. (C) Confocal imaging of native GFP expression in the area outside of the vascular arcade. (D) Confocal imaging of native GFP expression in a cryostat section through the fovea. (E) Native GFP expression in the inferior retina, outside the vascular arcade, shows little GFP expression in ganglion cells, but shows high levels of expression in Müller cells and some photoreceptors in the outer retina. Autofluorescence was also observed in RPE. (F) Anti-GFP labeling in a cryostat section revealed GFP expression in photoreceptors, as evidenced by their outer segments, Müller cells, and retina-spanning processes as well as cells in the inner nuclear layer with horizontal processes that are likely interneurons. (G) Anti-GFP labeling in a foveal section reveals additional infected cones, Müller glia, and interneurons. (H) Colabeling with anti–cone arrestin and anti-GFP antibodies reveals GFP expression in rod photoreceptors as well as cells in the inner nuclear layer, in a section taken next to the optic nerve head. (I) Colabeling with anti–cone arrestin and anti-GFP antibodies in an area of low expression reveals GFP expression in inner nuclear layer cells. (J and K) Montages of confocal images from cryostat sections collected outside the vascular arcade show efficient expression of GFP in the inner nuclear layer and outer retina. Scale bar: 200 μm (A and B), 100 μm (C–K). RPE, retinal pigment epithelium.