(A) Percentages of αβ and γδ T cells, NK cells, B cells, monocytes, myeloid dendritic cells (mDCs), and plasmacytoid dendritic cells (pDCs) in PBMCs are represented in dot plot graphs. Data were obtained from FACS analysis after cell-specific staining. Reference ranges for age-matched controls (3–18 years) correspond to 21%–98%, 0.9%–17%, 4%–22 %, 6%–24%, 2%–21%, 0.1%–7.8%, 0%–4.5%, 0.1%–3.1%, and 0.8%–0.36% for αβ T cells, γδ T cells, NK cells, B cells, classical CD14⁺⁺CD16^–, intermediate CD14⁺CD16⁺, nonclassical CD14⁺CD16⁺⁺ monocytes, mDCs, and pDCs, respectively. (B) Frequencies of naive (CD31⁺CD45RA⁺CCR7^–), central memory (CD45RA^–CCR7⁺CD27^–), effector memory (CD45RA^–CCR7⁺CD27^–), and exhausted effector memory/T_EMRA (CD45RA⁺CCR7⁺CD27^–) compartments in CD4⁺ and CD8⁺ T cells are represented in the dot plot graph. Representative dot plot FACS analysis of naive and memory CD4⁺ cells (left) and CD8⁺ cells (right) are also depicted. (C) Frequencies of senescent cells (CD8⁺CD57⁺) gated on CD3⁺ cells are shown in the dot plot graph (left). Representative dot plot FACS analysis of senescent CD8⁺ cell populations are depicted at right . Data were obtained from FACS analysis of cell-specific staining. (D) Frequencies of naive (CD19⁺CD27^–), memory (CD19⁺CD27⁺) (left), circulating marginal zone (MZ) (CD19⁺CD27⁺IgM⁺IgD⁺) and switched B cells (CD19⁺CD27⁺IgM^–IgD^–) (right) are shown in the dot plot graph. Representative dot plots FACS analysis of naive/memory (left) and marginal zone/switched (right) B cell populations are depicted. Data were obtained from FACS analysis after cell-specific staining. In A–D, black or red dots correspond to a healthy individual donor or a patient, respectively. Each circle represents an independent biological sample. Ten to 19 adult controls and 8 patients were analyzed. The horizontal bars represent the median. Data obtained from 6 independent experiments. Groups of values were compared 2 by 2 using Mann-Whitney U tests. *P < 0.05; **P < 0.01.