(A) Activated memory T cells were enumerated by flow cytometry from splenocytes purified from the different mouse lines at the ages indicated. Data are presented as box plots, where the boxes represent the 25th to 75th percentiles, the lines within the boxes represent the median, and the lines outside the boxes the 10th and 90th percentiles. Each circle represents an individual mouse. (B) Assessment of renal pathology in the different lines of mice. Data are presented as total kidney histology scores (KHSs) in the NZM.2328 mice with the indicated genotypes at the indicated ages. Each symbol represents an individual mouse. Results are plotted as in A. (C) Naive and activated memory T cells were stimulated with PMA and ionomycin for 5 hours and Brefeldin A (BFA) for 4 hours (day 0) or were stimulated with anti-CD3/CD28–coated beads for 5 days,and PMA + ionomycin were added to the culture for the last 5 hours as above and stained for intracellular IL-17A at day 0 and day 5. (D) Comparison between IL-17A production by activated memory T cells from age-matched NZM and C57BL/6 female mice stimulated as above. (E) IL-17A levels measured by commercial ELISA in supernatants from cultures of activated T cells isolated and stimulated as above. (C and D) Representative flow cytometry dot plots of 4 independent experiments are shown. (D and E) n = 5 per each group. (A–E) Only statistically significant differences are marked, based on using 1-way ANOVA followed by Tukey’s multiple-comparisons test (A and B) and unpaired Student’s t test (E). *P < 0.01, **P < 0.001, ***P < 0.0001.