Mice were injected with 200 μg MCA s.c. The injection site was harvested 2 weeks later (A–D and M–O) or the palpable tumor 9 and 18 weeks later (E–L and M–O) and analyzed by flow cytometry. Frequencies of CD3⁺ cells (A, E, and I), CD3^–NK1.1⁺ cells (B, F, and J), and CD11b⁺ and CD11c⁺ cells (C, G, and K) were measured. (D, H, and L) Remaining CD11b^–CD11c^– cells were further separated into populations that were either Ly6C⁺ or Gr1⁺. Each circle represents samples from a single mouse. The error bars depict standard deviation. The bar predicts the median. The length of the box represents the interquartile range. (M and N) The frequency of leukocytes (CD45⁺ cells) within the tumor was determined at each time point (M), as well as the frequency of lymphocytes (CD3⁺ and NK1.1⁺) among the CD45⁺ cells (N). (O) Flow cytometry (FCS) data were analyzed via unsupervised approaches using the MATLAB tool Cyt. The L1 statistical differences between the MFI distribution of the indicated markers on CD45⁺ cells were determined. The data are reported so that distributions derived from CD91^fl/flCD11c^Cre mice are the reference population, and the difference calculated is the change in the CD91^fl/fl mice distribution from the reference. Analyses were done on n = 6 mice/group, and statistical significance was determined by Student’s 2-tailed t test.