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Multiscale light-sheet for rapid imaging of cardiopulmonary system
Yichen Ding, … , René R. Sevag Packard, Tzung K. Hsiai
Yichen Ding, … , René R. Sevag Packard, Tzung K. Hsiai
Published August 23, 2018
Citation Information: JCI Insight. 2018;3(16):e121396. https://doi.org/10.1172/jci.insight.121396.
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Review

Multiscale light-sheet for rapid imaging of cardiopulmonary system

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Abstract

The ability to image tissue morphogenesis in real-time and in 3-dimensions (3-D) remains an optical challenge. The advent of light-sheet fluorescence microscopy (LSFM) has advanced developmental biology and tissue regeneration research. In this review, we introduce a LSFM system in which the illumination lens reshapes a thin light-sheet to rapidly scan across a sample of interest while the detection lens orthogonally collects the imaging data. This multiscale strategy provides deep-tissue penetration, high-spatiotemporal resolution, and minimal photobleaching and phototoxicity, allowing in vivo visualization of a variety of tissues and processes, ranging from developing hearts in live zebrafish embryos to ex vivo interrogation of the microarchitecture of optically cleared neonatal hearts. Here, we highlight multiple applications of LSFM and discuss several studies that have allowed better characterization of developmental and pathological processes in multiple models and tissues. These findings demonstrate the capacity of multiscale light-sheet imaging to uncover cardiovascular developmental and regenerative phenomena.

Authors

Yichen Ding, Jianguo Ma, Adam D. Langenbacher, Kyung In Baek, Juhyun Lee, Chih-Chiang Chang, Jeffrey J. Hsu, Rajan P. Kulkarni, John Belperio, Wei Shi, Sara Ranjbarvaziri, Reza Ardehali, Yin Tintut, Linda L. Demer, Jau-Nian Chen, Peng Fei, René R. Sevag Packard, Tzung K. Hsiai

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Figure 1

Fundamental concept of the light-sheet imaging strategy.

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Fundamental concept of the light-sheet imaging strategy.
(A) Crucial pro...
(A) Crucial procedures of multiscale imaging are indicated from embryonic zebrafish and rodent models. (B) The sample holder is oriented by a five-axis mounting stage for scanning the biological specimen. The laser light-sheet is excited from the illumination lenses (IL I and IL II) in a 2-D plane, which is orthogonal to the detection lens (DL). (C and D) A schematic and a photo illustrate the conversion of laser light to a sheet that can transversely illuminate a thin layer of the sample. (E) This photo depicts an array of laser beams aligned for multichannel fluorescent detection. (Reproduced with permission from ref. 68.)

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