Elevation of master autophagy regulator <i>Tfeb</i> in osteoblast lineage cells increases bone mass and strength

Alicen James; James A. Hendrixson; Ilham Kadhim; Adriana Marques-Carvalho; Jacob Laster; Julie Crawford; Jeff Thostenson; Visanu Wanchai; Amy Y. Sato; Intawat Nookaew; Jinhu Xiong; Maria Almeida; Melda Onal

doi:10.1172/jci.insight.191688

Elevation of master autophagy regulator Tfeb in osteoblast lineage cells increases bone mass and strength

Alicen James,¹ James A. Hendrixson,¹ Ilham Kadhim,¹ Adriana Marques-Carvalho,² Jacob Laster,¹ Julie Crawford,³ Jeff Thostenson,⁴ Visanu Wanchai,³ Amy Y. Sato,¹ Intawat Nookaew,¹ Jinhu Xiong,³ Maria Almeida,² and Melda Onal¹

Published July 29, 2025 - More info

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Abstract

Autophagy is a recycling pathway in which damaged proteins, protein aggregates, and organelles are delivered to lysosomes for degradation. Autophagy insufficiency is thought to contribute to osteoporosis. Accordingly, autophagy elimination from the osteoblast lineage reduces bone formation and bone mass. However, whether increasing autophagy would benefit bone health is unknown. Here, we increased expression of endogenous transcription factor EB gene (Tfeb) in osteoblast lineage cells in vivo via CRISPR activation (Tfeb^CRa mice). Elevated Tfeb stimulated autophagy and lysosomal biogenesis in osteoblasts. Tfeb^CRa mice displayed a robust increase in femoral and vertebral cortical thickness at 4.5 months of age. Increases in cortical thickness was due to increased periosteal bone formation. Tfeb elevation also increased femoral trabecular bone volume. These changes increased bone strength of Tfeb^CRa mice. Female Tfeb^CRa mice displayed a progressive increase in bone mass and at 12 months of age had high cortical thickness and trabecular bone volume. Increased vertebral trabecular bone volume was due to elevated bone formation. Osteoblastic cultures showed that Tfeb elevation increased proliferation and mineral deposition. Overall, these results demonstrate TFEB-driven stimulation of autophagy in osteoblast lineage cells is associated with increased bone formation and strength and may represent an effective approach to combat osteoporosis.

Elevation of master autophagy regulator Tfeb in osteoblast lineage cells increases bone mass and strength

Alicen James,¹ James A. Hendrixson,¹ Ilham Kadhim,¹ Adriana Marques-Carvalho,² Jacob Laster,¹ Julie Crawford,³ Jeff Thostenson,⁴ Visanu Wanchai,³ Amy Y. Sato,¹ Intawat Nookaew,¹ Jinhu Xiong,³ Maria Almeida,² and Melda Onal¹

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Elevation of master autophagy regulator Tfeb in osteoblast lineage cells increases bone mass and strength

Alicen James,1 James A. Hendrixson,1 Ilham Kadhim,1 Adriana Marques-Carvalho,2 Jacob Laster,1 Julie Crawford,3 Jeff Thostenson,4 Visanu Wanchai,3 Amy Y. Sato,1 Intawat Nookaew,1 Jinhu Xiong,3 Maria Almeida,2 and Melda Onal1

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Alicen James,¹ James A. Hendrixson,¹ Ilham Kadhim,¹ Adriana Marques-Carvalho,² Jacob Laster,¹ Julie Crawford,³ Jeff Thostenson,⁴ Visanu Wanchai,³ Amy Y. Sato,¹ Intawat Nookaew,¹ Jinhu Xiong,³ Maria Almeida,² and Melda Onal¹