[CITATION][C] [65] Determination of the true immunoreactive fraction of monoclonal antibodies after radiolabeling
T Lindmo, PA Bunn Jr - Methods in enzymology, 1986 - Elsevier
Conclusion Acylation with N-succinimidyl [2, 3-3H] propionate is a highly specific method to
radiolabel proteins under relatively mild conditions. This method of linkage produces
conjugates with specific activities approaching those obtained with radioiodination. The
labeled proteins retain excellent functional activity at the level of modification presented
here. In addition, neither the labeling procedure nor conjugate purification requires special
expertise. 8
radiolabel proteins under relatively mild conditions. This method of linkage produces
conjugates with specific activities approaching those obtained with radioiodination. The
labeled proteins retain excellent functional activity at the level of modification presented
here. In addition, neither the labeling procedure nor conjugate purification requires special
expertise. 8