Cellular cholesterol enrichment impairs T cell activation and chemotaxis
DH Nguyen, JC Espinoza, DD Taub - Mechanisms of ageing and …, 2004 - Elsevier
Human aging is associated with an increase in immune cell cholesterol levels, independent
of circulating cholesterol levels. The effects of such an increase in membrane cholesterol on
lipid raft-associated immune cell function have not been investigated. We sought to examine
the effects of in vitro cholesterol loading on two known lipid raft-associated pathways of T
cells, namely T cell activation and chemokine stimulation. Using β-cyclodextrin (BCD) as a
vehicle, we were able to rapidly load cholesterol onto human T cell lines and primary …
of circulating cholesterol levels. The effects of such an increase in membrane cholesterol on
lipid raft-associated immune cell function have not been investigated. We sought to examine
the effects of in vitro cholesterol loading on two known lipid raft-associated pathways of T
cells, namely T cell activation and chemokine stimulation. Using β-cyclodextrin (BCD) as a
vehicle, we were able to rapidly load cholesterol onto human T cell lines and primary …