[HTML][HTML] RNA sequence elements required for high affinity binding by the zinc finger domain of tristetraprolin: conformational changes coupled to the bipartite nature of …
BY Brewer, J Malicka, PJ Blackshear… - Journal of Biological …, 2004 - ASBMB
Tristetraprolin (TTP) binds AU-rich elements (AREs) encoded within selected labile mRNAs
and targets these transcripts for rapid cytoplasmic decay. RNA binding by TTP is mediated
by an∼ 70-amino acid domain containing two tandemly arrayed CCCH zinc fingers. Here
we show that a 73-amino acid peptide spanning the TTP zinc finger domain, denoted
TTP73, forms a dynamic, equimolar RNA· peptide complex with a 13-nucleotide fragment of
the ARE from tumor necrosis factor α mRNA, which includes small but significant …
and targets these transcripts for rapid cytoplasmic decay. RNA binding by TTP is mediated
by an∼ 70-amino acid domain containing two tandemly arrayed CCCH zinc fingers. Here
we show that a 73-amino acid peptide spanning the TTP zinc finger domain, denoted
TTP73, forms a dynamic, equimolar RNA· peptide complex with a 13-nucleotide fragment of
the ARE from tumor necrosis factor α mRNA, which includes small but significant …