Granulocyte macrophage colony-stimulating factor improves survival in two models of gut-derived sepsis by improving gut barrier function and modulating bacterial …
R Gennari, JW Alexander, L Gianotti… - Annals of …, 1994 - journals.lww.com
R Gennari, JW Alexander, L Gianotti, T Eaves-Pyles, S Hartmann
Annals of surgery, 1994•journals.lww.comObjective: The effect of recombinant murine granulocyte macrophage colony-stimulating
factor (rmGM-CSF) on survival and host defense was studied using two clinically relevant
models of infection that included transfusion-induced immunosuppression. Summary
Background Data: Granulocyte macrophage colony-stimulating factor improves resistance in
several models of infection, but its role in transfusion-induced immunosuppression and
bacterial translocation (gut-derived sepsis) has not been defined. Methods: Balb/c mice …
factor (rmGM-CSF) on survival and host defense was studied using two clinically relevant
models of infection that included transfusion-induced immunosuppression. Summary
Background Data: Granulocyte macrophage colony-stimulating factor improves resistance in
several models of infection, but its role in transfusion-induced immunosuppression and
bacterial translocation (gut-derived sepsis) has not been defined. Methods: Balb/c mice …
Abstract
Objective: The effect of recombinant murine granulocyte macrophage colony-stimulating factor (rmGM-CSF) on survival and host defense was studied using two clinically relevant models of infection that included transfusion-induced immunosuppression.
Summary Background Data: Granulocyte macrophage colony-stimulating factor improves resistance in several models of infection, but its role in transfusion-induced immunosuppression and bacterial translocation (gut-derived sepsis) has not been defined.
Methods: Balb/c mice were treated with 100 ng of rmGM-CSF or placebo for 6 days in a model of transfusion, burn, and gavage, or cecal ligation and puncture (CLP). Translocation was studied in the first model.
Results: Survival after transfusion, burn, and gavage was 90% in rmGM-CSF-treated animals versus 35% in the control group (p< 0.001). After CLP, survival was 75% in the rmGM-CSF group versus 30% in the control group (p= 0.01). Less translocation and better killing of bacteria was observed in the tissues in animals treated with rmGM-CSF.
Conclusion: The ability of rmGM-CSF to improve gut barrier function and enhance killing of translocated organisms after burn injury-induced gut origin sepsis was associated with improved outcome. Granulocyte macrophage colony-stimulating factor also improved survival after CLP.
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