[HTML][HTML] Bone overgrowth-associated mutations in the LRP4 gene impair sclerostin facilitator function
O Leupin, E Piters, C Halleux, S Hu, I Kramer… - Journal of Biological …, 2011 - ASBMB
Humans lacking sclerostin display progressive bone overgrowth due to increased bone
formation. Although it is well established that sclerostin is an osteocyte-secreted bone
formation inhibitor, the underlying molecular mechanisms are not fully elucidated. We
identified in tandem affinity purification proteomics screens LRP4 (low density lipoprotein-
related protein 4) as a sclerostin interaction partner. Biochemical assays with recombinant
proteins confirmed that sclerostin LRP4 interaction is direct. Interestingly, in vitro …
formation. Although it is well established that sclerostin is an osteocyte-secreted bone
formation inhibitor, the underlying molecular mechanisms are not fully elucidated. We
identified in tandem affinity purification proteomics screens LRP4 (low density lipoprotein-
related protein 4) as a sclerostin interaction partner. Biochemical assays with recombinant
proteins confirmed that sclerostin LRP4 interaction is direct. Interestingly, in vitro …