Wnt/β-catenin signaling is initiated at the cell surface by association of secreted Wnt with its
receptors Frizzled (Fz) and low density lipoprotein receptor-related protein 5/6 (LRP5/6). The
study of these molecular interactions has been a significant technical challenge because the
proteins have been inaccessible in sufficient purity and quantity. In this report we describe
insect cell expression and purification of soluble mouse Fz8 cysteine-rich domain and
human LRP6 extracellular domain and show that they inhibit Wnt/β-catenin signaling in …