CD83 expression is a sensitive marker of activation required for B cell and CD4+ T cell longevity in vivo

CM Prazma, N Yazawa, Y Fujimoto… - The Journal of …, 2007 - journals.aai.org
CM Prazma, N Yazawa, Y Fujimoto, M Fujimoto, TF Tedder
The Journal of Immunology, 2007journals.aai.org
CD83 is a surface marker that differentiates immature and mature human dendritic cell
populations. Thymic epithelial cell expression of CD83 is also necessary for efficient CD4+ T
cell development in mice. The altered phenotypes of peripheral B and CD4+ T cells, and the
reduction of peripheral CD4+ T cells in CD83−/− mice, suggest additional functions for
CD83. To assess this, a panel of mAbs was generated to characterize mouse CD83
expression by peripheral leukocytes. As in humans, activation of conventional and …
Abstract
CD83 is a surface marker that differentiates immature and mature human dendritic cell populations. Thymic epithelial cell expression of CD83 is also necessary for efficient CD4+ T cell development in mice. The altered phenotypes of peripheral B and CD4+ T cells, and the reduction of peripheral CD4+ T cells in CD83−/− mice, suggest additional functions for CD83. To assess this, a panel of mAbs was generated to characterize mouse CD83 expression by peripheral leukocytes. As in humans, activation of conventional and plasmacytoid murine dendritic cell subsets led to rapid up-regulation of CD83 surface expression in mice. In primary and secondary lymphoid compartments, a subset of B cells expressed low-level CD83, while CD83 was not detected on resting T cells. However, CD83 was prominently up-regulated on the majority of spleen B and T cells within hours of activation in vitro. In vivo, a low dose of hen egg lysozyme (1 μg) induced significant CD83 but not CD69 expression by Ag-specific B cells within 4 h of Ag challenge. Although B cell development appeared normal in CD83−/− mice, B and CD4+ T cell expression of CD83 was required for lymphocyte longevity in adoptive transfer experiments. Thus, the restricted expression pattern of CD83, its rapid induction following B cell and T cell activation, and its requirement for B cell and CD4+ T cell longevity demonstrate that CD83 is a functionally significant and sensitive marker of early lymphocyte activation in vivo.
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