The nature of protein kinase C activation by physically defined phospholipid vesicles and diacylglycerols.
LT Boni, RR Rando - Journal of Biological Chemistry, 1985 - ASBMB
Protein kinase C is activated by a 1, 2-sn-diacylglycerol and phospholipid at low calcium
concentrations. Of the various phospholipids studied, phosphatidylserine has been shown to
be the most effective one and is usually used in assaying the enzyme (Kaibuchi, K., Takai,
Y., and Nishizuka, Y.(1981) J. Biol. Chem. 256, 7146-7149). It is shown here that under the
conditions of the enzymatic assay, phosphatidylserine does not form typical fluid bilayer
structures as seen by electron microscopy and fluorescence polarization. On the other hand …
concentrations. Of the various phospholipids studied, phosphatidylserine has been shown to
be the most effective one and is usually used in assaying the enzyme (Kaibuchi, K., Takai,
Y., and Nishizuka, Y.(1981) J. Biol. Chem. 256, 7146-7149). It is shown here that under the
conditions of the enzymatic assay, phosphatidylserine does not form typical fluid bilayer
structures as seen by electron microscopy and fluorescence polarization. On the other hand …