[HTML][HTML] Evidence for shear-mediated Ca2+ entry through mechanosensitive cation channels in human platelets and a megakaryocytic cell line
The role of mechanosensitive (MS) Ca 2+-permeable ion channels in platelets is unclear,
despite the importance of shear stress in platelet function and life-threatening thrombus
formation. We therefore sought to investigate the expression and functional relevance of MS
channels in human platelets. The effect of shear stress on Ca 2+ entry in human platelets
and Meg-01 megakaryocytic cells loaded with Fluo-3 was examined by confocal
microscopy. Cells were attached to glass coverslips within flow chambers that allowed …
despite the importance of shear stress in platelet function and life-threatening thrombus
formation. We therefore sought to investigate the expression and functional relevance of MS
channels in human platelets. The effect of shear stress on Ca 2+ entry in human platelets
and Meg-01 megakaryocytic cells loaded with Fluo-3 was examined by confocal
microscopy. Cells were attached to glass coverslips within flow chambers that allowed …