Circulating cells contribute to cardiomyocyte regeneration after injury
Circulation research, 2015•Am Heart Assoc
Rationale: The contribution of bone marrow–borne hematopoietic cells to the ischemic
myocardium has been documented. However, a pivotal study reported no evidence of
myocardial regeneration from hematopoietic-derived cells. The study did not take into
account the possible effect of early injury–induced signaling as the test mice were
parabiotically paired to partners immediately after surgery-induced myocardial injury when
cross-circulation has not yet developed. Objective: To re-evaluate the role of circulating cells …
myocardium has been documented. However, a pivotal study reported no evidence of
myocardial regeneration from hematopoietic-derived cells. The study did not take into
account the possible effect of early injury–induced signaling as the test mice were
parabiotically paired to partners immediately after surgery-induced myocardial injury when
cross-circulation has not yet developed. Objective: To re-evaluate the role of circulating cells …
Rationale:
The contribution of bone marrow–borne hematopoietic cells to the ischemic myocardium has been documented. However, a pivotal study reported no evidence of myocardial regeneration from hematopoietic-derived cells. The study did not take into account the possible effect of early injury–induced signaling as the test mice were parabiotically paired to partners immediately after surgery-induced myocardial injury when cross-circulation has not yet developed.
Objective:
To re-evaluate the role of circulating cells in the injured myocardium.
Methods and Results:
By combining pulse-chase labeling and parabiosis model, we show that circulating cells derived from the parabiont expressed cardiac-specific markers in the injured myocardium. Genetic fate mapping also revealed that circulating hematopoietic cells acquired cardiac cell fate by means of cell fusion and transdifferentiation.
Conclusions:
These results suggest that circulating cells participate in cardiomyocyte regeneration in a mouse model of parabiosis when the circulatory system is fully developed before surgery-induced heart injury.
Am Heart Assoc