Selectivity of tissue expression for surface molecules of cells lining the vasculature has been uncovered in disease and during normal development. Systematic profiling of selectively expressed “vascular addresses” has revealed prospective molecular targets that may be used to direct therapies to specific tissues. In vivo phage display is a technology used to reveal organ-specific vascular ligand-receptor systems in animal models and, recently, in patients, and to validate them as potential therapy targets. Because a single biopanning round of a phage library may not always sufficiently enrich for organ-homing peptides, until now, phage display screens have encompassed recovery of phage from the organ of interest in three to four rounds of library selection, utilizing one subject per each round. Thus, systematic characterization of the vasculature by using this “conventional” in vivo phage display screen setup has been rate-limited by the need to separately perform individual multiround screens for motifs homing to each tissue studied. To screen phage libraries in patients more efficiently, we have initiated attempts to isolate tissue-homing peptides for several tissues in a single screen. Here, we integrated a comprehensive strategy to simultaneously screen phage display libraries for peptides homing to any number of tissues without the need for an individual subject for each target tissue.