[HTML][HTML] CFTR-mutation specific applications of CFTR-directed monoclonal antibodies
MA Van Meegen, SWJ Terheggen, KJ Koymans… - Journal of Cystic …, 2013 - Elsevier
MA Van Meegen, SWJ Terheggen, KJ Koymans, LAW Vijftigschild, JF Dekkers…
Journal of Cystic Fibrosis, 2013•ElsevierBackground Over the last decade novel monoclonal CFTR-specific antibodies have been
developed. We here present a paired analysis to detect wild-type and mutant CFTR using
Western blot analysis, flow cytometry and confocal microscopy in several cellular expression
systems. Methods The following CFTR-specific antibodies were used; 217, 432, 450, 570,
769, 596, 660, L12B4 and 24.1. Mutant CFTR was detected in HEK293 cells transiently
expressing the mutations; G542X, R1162X, F508del, N1303K, G551D, R117H, A455E …
developed. We here present a paired analysis to detect wild-type and mutant CFTR using
Western blot analysis, flow cytometry and confocal microscopy in several cellular expression
systems. Methods The following CFTR-specific antibodies were used; 217, 432, 450, 570,
769, 596, 660, L12B4 and 24.1. Mutant CFTR was detected in HEK293 cells transiently
expressing the mutations; G542X, R1162X, F508del, N1303K, G551D, R117H, A455E …
Background
Over the last decade novel monoclonal CFTR-specific antibodies have been developed. We here present a paired analysis to detect wild-type and mutant CFTR using Western blot analysis, flow cytometry and confocal microscopy in several cellular expression systems.
Methods
The following CFTR-specific antibodies were used; 217, 432, 450, 570, 769, 596, 660, L12B4 and 24.1. Mutant CFTR was detected in HEK293 cells transiently expressing the mutations; G542X, R1162X, F508del, N1303K, G551D, R117H, A455E.
Results
The majority of these antibodies are suitable for most applications tested. Using immunofluorescence, some antibodies can better detect mutant forms of CFTR (F508del and N1303K by mAbs 596 and 769), or display lower aspecific detection by Western blot analysis (mAbs 432, 450, 769 and 596) or immunofluorescence (mAbs 432, 450, 570 and 769).
Conclusion
Optimal detection of CFTR by monoclonal antibodies depends on CFTR mutation and the specific research application.
Elsevier