Synthesis of bile acid coenzyme a thioesters in the amino acid conjugation of bile acids
EM Shonsey, J Wheeler, M Johnson, D He… - Methods in …, 2005 - Elsevier
Bile acids are converted to their glycine and taurine N‐acyl amidates by enzymes in the liver
in a two‐step process. This conjugation reaction increases the aqueous solubility of bile
acids, particularly in the acidic environment of the initial portion of the small intestine. In the
first step, bile acid coenzyme A (CoA) thioesters are formed by a bile acid CoA ligase (BAL).
This chapter describes the methods used to purify BAL from rat liver microsomes and to
isolate and clone the cDNAs encoding BAL from a rat liver cDNA library, the expression of …
in a two‐step process. This conjugation reaction increases the aqueous solubility of bile
acids, particularly in the acidic environment of the initial portion of the small intestine. In the
first step, bile acid coenzyme A (CoA) thioesters are formed by a bile acid CoA ligase (BAL).
This chapter describes the methods used to purify BAL from rat liver microsomes and to
isolate and clone the cDNAs encoding BAL from a rat liver cDNA library, the expression of …