An impedance-based cytotoxicity assay for real-time and label-free assessment of T-cell-mediated killing of adherent cells
JK Peper, H Schuster, MW Löffler… - Journal of …, 2014 - Elsevier
The in vitro assessment of T-cell-mediated cytotoxicity plays an important and increasingly
relevant role both in preclinical target evaluation and during immunomonitoring to
accompany clinical trials employing targeted immunotherapies. For a long time, the gold
standard for this purpose has been the chromium release assay (CRA). This end point
assay, however, shows several disadvantages including the inevitable use of radioactivity.
Based on electrical impedance measurements (using the xCELLigence system), we have …
relevant role both in preclinical target evaluation and during immunomonitoring to
accompany clinical trials employing targeted immunotherapies. For a long time, the gold
standard for this purpose has been the chromium release assay (CRA). This end point
assay, however, shows several disadvantages including the inevitable use of radioactivity.
Based on electrical impedance measurements (using the xCELLigence system), we have …