Dual functions of fractalkine/CX3C ligand 1 in trafficking of perforin+/granzyme B+ cytotoxic effector lymphocytes that are defined by CX3CR1 expression

M Nishimura, H Umehara, T Nakayama… - The Journal of …, 2002 - journals.aai.org
M Nishimura, H Umehara, T Nakayama, O Yoneda, K Hieshima, M Kakizaki, N Dohmae…
The Journal of immunology, 2002journals.aai.org
Fractalkine/CX3C ligand 1 and its receptor CX3CR1 are known to mediate both cell
adhesion and cell migration. Here we show that CX3CR1 defines peripheral blood cytotoxic
effector lymphocytes commonly armed with intracellular perforin and granzyme B, which
include NK cells, γδ T cells, and terminally differentiated CD8+ T cells. In addition, soluble
fractalkine preferentially induced migration of cytotoxic effector lymphocytes. Furthermore,
interaction of cytotoxic effector lymphocytes with membrane-bound fractalkine promoted …
Abstract
Fractalkine/CX3C ligand 1 and its receptor CX3CR1 are known to mediate both cell adhesion and cell migration. Here we show that CX3CR1 defines peripheral blood cytotoxic effector lymphocytes commonly armed with intracellular perforin and granzyme B, which include NK cells, γδ T cells, and terminally differentiated CD8+ T cells. In addition, soluble fractalkine preferentially induced migration of cytotoxic effector lymphocytes. Furthermore, interaction of cytotoxic effector lymphocytes with membrane-bound fractalkine promoted subsequent migration to the secondary chemokines, such as macrophage inflammatory protein-1β/CC ligand 4 or IL-8/CXC ligand 8. Thus, fractalkine expressed on inflamed endothelium may function as a vascular regulator for cytotoxic effector lymphocytes, regardless of their lineage and mode of target cell recognition, through its ability to capture them from blood flow and to promote their emigration in response to other chemokines.
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