[HTML][HTML] High fat diet impairs the function of glucagon-like peptide-1 producing L-cells
Abstract Glucagon-like peptide-1 (GLP-1) acts as a satiety signal and enhances insulin
release. This study examined how GLP-1 production from intestinal L-cells is modified by
dietary changes. Methods Transgenic mouse models were utilized in which L-cells could be
purified by cell specific expression of a yellow fluorescent protein, Venus. Mice were fed on
chow or 60% high fat diet (HFD) for 2 or 16 weeks. L-cells were purified by flow cytometry
and analysed by microarray and quantitative RT-PCR. Enteroendocrine cell populations …
release. This study examined how GLP-1 production from intestinal L-cells is modified by
dietary changes. Methods Transgenic mouse models were utilized in which L-cells could be
purified by cell specific expression of a yellow fluorescent protein, Venus. Mice were fed on
chow or 60% high fat diet (HFD) for 2 or 16 weeks. L-cells were purified by flow cytometry
and analysed by microarray and quantitative RT-PCR. Enteroendocrine cell populations …