Purification and cloning of aggrecanase-1: a member of the ADAMTS family of proteins

MD Tortorella, TC Burn, MA Pratta, I Abbaszade… - Science, 1999 - science.org
MD Tortorella, TC Burn, MA Pratta, I Abbaszade, JM Hollis, R Liu, SA Rosenfeld…
Science, 1999science.org
We purified, cloned, and expressed aggrecanase, a protease that is thought to be
responsible for the degradation of cartilage aggrecan in arthritic diseases. Aggrecanase-1 [a
disintegrin and metalloproteinase with thrombospondin motifs–4 (ADAMTS-4)] is a member
of the ADAMTS protein family that cleaves aggrecan at the glutamic acid-373–alanine-374
bond. The identification of this protease provides a specific target for the development of
therapeutics to prevent cartilage degradation in arthritis.
We purified, cloned, and expressed aggrecanase, a protease that is thought to be responsible for the degradation of cartilage aggrecan in arthritic diseases. Aggrecanase-1 [a disintegrin and metalloproteinase with thrombospondin motifs–4 (ADAMTS-4)] is a member of the ADAMTS protein family that cleaves aggrecan at the glutamic acid-373–alanine-374 bond. The identification of this protease provides a specific target for the development of therapeutics to prevent cartilage degradation in arthritis.
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