Repression of a malignant cell-substratum adhesion phenotype by inhibiting the production of the anti-adhesive proteoglycan, PG-M/versican

M Yamagata, K Kimata - Journal of Cell Science, 1994 - journals.biologists.com
M Yamagata, K Kimata
Journal of Cell Science, 1994journals.biologists.com
Malignantly transformed cells usually display a rosette-like morphology of substratum
adhesions (called podosomes) and disorganized microfilaments, and are often associated
with elevated production of chondroitin sulphate. We pre-viously showed that many tissues
and cells express alter-natively spliced multiforms of the large chondroitin sulphate
proteoglycan termed PG-M (versican is one of the short transcripts). Since PG-M/versican
inhibits many types of cell-substratum adhesion and is found to be excluded from focal …
Abstract
Malignantly transformed cells usually display a rosette-like morphology of substratum adhesions (called podosomes) and disorganized microfilaments, and are often associated with elevated production of chondroitin sulphate. We pre-viously showed that many tissues and cells express alter-natively spliced multiforms of the large chondroitin sulphate proteoglycan termed PG-M (versican is one of the short transcripts). Since PG-M/versican inhibits many types of cell-substratum adhesion and is found to be excluded from focal contacts of cultured fibroblasts, it is likely that this proteoglycan is generally involved in regulating cell-substratum adhesion. We report here that PG-M/versican is selectively excluded from podosomes of human osteosarcoma cells and that specific inhibition of its biosynthesis by an antisense method suppresses such a malignant cell-adhesive phenotype. The results support the idea that PG-M/versican acts as an anti-adhesive molecule and raise the possibility that PG-M/versican controls one type of cancer cell behaviour.
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