Potential risk of replication-competent virus in HIV-1 Env-pseudotyped virus preparations
M Bilska, H Tang, DC Montefiori - AIDS Research and Human …, 2017 - liebertpub.com
M Bilska, H Tang, DC Montefiori
AIDS Research and Human Retroviruses, 2017•liebertpub.comEnv-pseudotyped viruses are valuable reagents for studies of HIV-1 neutralizing antibodies.
It is often assumed that all pseudovirus particles are capable of only a single round of
infection, making them a safe alternative to work with live HIV-1. In this study, we show that
some Env-pseudotyped virus preparations give rise to low levels of replication-competent
virus. These levels did not compromise results in the TZM-bl neutralization assay; however,
their presence highlights a need to adhere to the same level of biosafety when working with …
It is often assumed that all pseudovirus particles are capable of only a single round of
infection, making them a safe alternative to work with live HIV-1. In this study, we show that
some Env-pseudotyped virus preparations give rise to low levels of replication-competent
virus. These levels did not compromise results in the TZM-bl neutralization assay; however,
their presence highlights a need to adhere to the same level of biosafety when working with …
Abstract
Env-pseudotyped viruses are valuable reagents for studies of HIV-1 neutralizing antibodies. It is often assumed that all pseudovirus particles are capable of only a single round of infection, making them a safe alternative to work with live HIV-1. In this study, we show that some Env-pseudotyped virus preparations give rise to low levels of replication-competent virus. These levels did not compromise results in the TZM-bl neutralization assay; however, their presence highlights a need to adhere to the same level of biosafety when working with Env-pseudotyped viruses that are required for work with replication competent HIV-1.
Mary Ann Liebert