Localization of HLA-ABC and DR antigens in human kidney

DNJ Hart, SV Fuggle, KA Williams, JW Fabre… - …, 1981 - journals.lww.com
DNJ Hart, SV Fuggle, KA Williams, JW Fabre, A Ting, PJ Morris
Transplantation, 1981journals.lww.com
Monoclonal antibodies to human monomorphic class I and class II major histocompatibility
complex (MHC) determinants have been used with immunofluorescence and
immunoperoxidase techniques, to localize these antigens in normal human kidneys. HLA-
DR antigen was located in the glomeruli (probably on endothelium as well as in the
mesangium) and within the cells of cortical and medullary tubules. Dendritic cells in the
renal inter-stitium stained brightly for the DR antigen and could be distinguished from the …
Abstract
Monoclonal antibodies to human monomorphic class I and class II major histocompatibility complex (MHC) determinants have been used with immunofluorescence and immunoperoxidase techniques, to localize these antigens in normal human kidneys. HLA-DR antigen was located in the glomeruli (probably on endothelium as well as in the mesangium) and within the cells of cortical and medullary tubules. Dendritic cells in the renal inter-stitium stained brightly for the DR antigen and could be distinguished from the staining of capillary endothelium. The vascular endothelium of large vessels stained less densely for the HLA-DR antigen than for HLA-ABC antigens. The glomeruli stained intensely for the HLA-ABC antigens and diffuse staining of HLA-ABC antigens was also noted within renal tubular cells.
Lippincott Williams & Wilkins