[HTML][HTML] Real-time quantification of fatty acid uptake using a novel fluorescence assay
J Liao, R Sportsman, J Harris, A Stahl - Journal of lipid research, 2005 - ASBMB
Uptake of nonesterified long-chain fatty acids (LCFAs) into many cell types and organs such
as liver, heart, intestine, and skeletal muscle occurs primarily through a saturable, protein-
mediated mechanism. Membrane proteins that increase the uptake of LCFAs, such as
FAT/CD36 and fatty acid transport proteins, represent significant therapeutic targets for the
treatment of metabolic disorders, including type 2 diabetes. However, currently available
methods for the quantification of LCFA uptake neither allow for real-time measurements of …
as liver, heart, intestine, and skeletal muscle occurs primarily through a saturable, protein-
mediated mechanism. Membrane proteins that increase the uptake of LCFAs, such as
FAT/CD36 and fatty acid transport proteins, represent significant therapeutic targets for the
treatment of metabolic disorders, including type 2 diabetes. However, currently available
methods for the quantification of LCFA uptake neither allow for real-time measurements of …