Subcellular fractionation of polarized epithelial cells and identification of organelle‐specific proteins by two‐dimensional gel electrophoresis

I Fialka, C Pasquali, F Lottspeich, H Ahorn… - …, 1997 - Wiley Online Library
I Fialka, C Pasquali, F Lottspeich, H Ahorn, LA Huber
Electrophoresis, 1997Wiley Online Library
Nonstandard abbreviations: EE, early endosomes; EpH4, murine mammary epithelial cell
line; FCS, fetal calf serum: GalT, fi-1, 4-galactosyltransferase; GRAVY, grand average or
hydropathicity; HB, homogenization buffer; HRP, horseradish peroxidase; LE, late
endosomes; PM, plasma membranc; RER and SER, rough and smooth endoplasmic
reliculum; TGN, trans-Golgi network
Nonstandard abbreviations: EE, early endosomes; EpH4, murine mammary epithelial cell line; FCS, fetal calf serum: GalT, fi-1, 4-galactosyltransferase; GRAVY, grand average or hydropathicity; HB, homogenization buffer; HRP, horseradish peroxidase; LE, late endosomes; PM, plasma membranc; RER and SER, rough and smooth endoplasmic reliculum; TGN, trans-Golgi network
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