Multiple apolipoprotein kinetics measured in human HDL by high-resolution/accurate mass parallel reaction monitoring [S]
SA Singh, AB Andraski, B Pieper, W Goh… - Journal of lipid …, 2016 - jlr.org
Endogenous labeling with stable isotopes is used to study the metabolism of proteins in
vivo. However, traditional detection methods such as GC/MS cannot measure tracer
enrichment in multiple proteins simultaneously, and multiple reaction monitoring MS cannot
measure precisely the low tracer enrichment in slowly turning-over proteins as in HDL. We
exploited the versatility of the high-resolution/accurate mass (HR/AM) quadrupole Orbitrap
for proteomic analysis of five HDL sizes. We identified 58 proteins in HDL that were shared …
vivo. However, traditional detection methods such as GC/MS cannot measure tracer
enrichment in multiple proteins simultaneously, and multiple reaction monitoring MS cannot
measure precisely the low tracer enrichment in slowly turning-over proteins as in HDL. We
exploited the versatility of the high-resolution/accurate mass (HR/AM) quadrupole Orbitrap
for proteomic analysis of five HDL sizes. We identified 58 proteins in HDL that were shared …
