Quantitative measurement of relative retinoic acid levels in E8. 5 embryos and neurosphere cultures using the F9 RARE-Lacz cell-based reporter assay
MR Ababon, BI Li, PG Matteson, JH Millonig - JoVE (Journal of Visualized …, 2016 - jove.com
Retinoic acid (RA) is an important developmental morphogen that coordinates
anteroposterior and dorsoventral axis patterning, somitic differentiation, neurogenesis,
patterning of the hindbrain and spinal cord, and the development of multiple organ systems.
Due to its chemical nature as a small amphipathic lipid, direct detection and visualization of
RA histologically remains technically impossible. Currently, methods used to infer the
presence and localization of RA make use of reporter systems that detect the biological …
anteroposterior and dorsoventral axis patterning, somitic differentiation, neurogenesis,
patterning of the hindbrain and spinal cord, and the development of multiple organ systems.
Due to its chemical nature as a small amphipathic lipid, direct detection and visualization of
RA histologically remains technically impossible. Currently, methods used to infer the
presence and localization of RA make use of reporter systems that detect the biological …