[HTML][HTML] Phosphodiesterase 4D forms a cAMP diffusion barrier at the apical membrane of the airway epithelium
AP Barnes, G Livera, P Huang, C Sun… - Journal of Biological …, 2005 - ASBMB
We demonstrated previously that Calu-3 airway epithelial cells sense adenosine on their
luminal surface through adenosine A2B receptors coupled to adenylyl cyclase. Occupancy
of these receptors leads to activation of the cystic fibrosis transmembrane conductance
regulator (CFTR) chloride channel through protein kinase A (PKA) anchored at the apical
membrane. Because luminal A2B receptor activation does not raise total cellular cAMP
levels, we hypothesized that activation of phosphodiesterases (PDEs) confines cAMP …
luminal surface through adenosine A2B receptors coupled to adenylyl cyclase. Occupancy
of these receptors leads to activation of the cystic fibrosis transmembrane conductance
regulator (CFTR) chloride channel through protein kinase A (PKA) anchored at the apical
membrane. Because luminal A2B receptor activation does not raise total cellular cAMP
levels, we hypothesized that activation of phosphodiesterases (PDEs) confines cAMP …