[HTML][HTML] A role for ceramides, but not sphingomyelins, as antagonists of insulin signaling and mitochondrial metabolism in C2C12 myotubes
M Park, V Kaddai, J Ching, KT Fridianto, RJ Sieli… - Journal of Biological …, 2016 - ASBMB
The accumulation of sphingolipids in obesity leads to impairments in insulin sensitivity and
mitochondrial metabolism, but the precise species driving these defects is unclear. We have
modeled these obesity-induced effects in cultured C2C12 myotubes, using BSA-conjugated
palmitate to increase synthesis of endogenous sphingolipids and to inhibit insulin signaling
and oxidative phosphorylation. Palmitate (a) induced the accumulation of sphingomyelin
(SM) precursors such as sphinganine, dihydroceramide, and ceramide;(b) inhibited insulin …
mitochondrial metabolism, but the precise species driving these defects is unclear. We have
modeled these obesity-induced effects in cultured C2C12 myotubes, using BSA-conjugated
palmitate to increase synthesis of endogenous sphingolipids and to inhibit insulin signaling
and oxidative phosphorylation. Palmitate (a) induced the accumulation of sphingomyelin
(SM) precursors such as sphinganine, dihydroceramide, and ceramide;(b) inhibited insulin …