[HTML][HTML] Evaluation of a systems biology approach to identify pharmacological correctors of the mutant CFTR chloride channel
E Pesce, G Gorrieri, F Sirci, F Napolitano… - Journal of Cystic …, 2016 - Elsevier
Journal of Cystic Fibrosis, 2016•Elsevier
Background Mistrafficking of CFTR protein caused by F508del, the most frequent mutation in
cystic fibrosis (CF), can be corrected by cell incubation at low temperature, an effect that may
be mediated by altered expression of proteostasis genes. Methods To identify small
molecules mimicking low temperature, we compared gene expression profiles of cells kept
at 27° C with those previously generated from more than 1300 compounds. The resulting
candidates were tested with a functional assay on a bronchial epithelial cell line. Results We …
cystic fibrosis (CF), can be corrected by cell incubation at low temperature, an effect that may
be mediated by altered expression of proteostasis genes. Methods To identify small
molecules mimicking low temperature, we compared gene expression profiles of cells kept
at 27° C with those previously generated from more than 1300 compounds. The resulting
candidates were tested with a functional assay on a bronchial epithelial cell line. Results We …
Background
Mistrafficking of CFTR protein caused by F508del, the most frequent mutation in cystic fibrosis (CF), can be corrected by cell incubation at low temperature, an effect that may be mediated by altered expression of proteostasis genes.
Methods
To identify small molecules mimicking low temperature, we compared gene expression profiles of cells kept at 27 °C with those previously generated from more than 1300 compounds. The resulting candidates were tested with a functional assay on a bronchial epithelial cell line.
Results
We found that anti-inflammatory glucocorticoids, such as mometasone, budesonide, and fluticasone, increased mutant CFTR function. However, this activity was not confirmed in primary bronchial epithelial cells. Actually, glucocorticoids enhanced Na+ absorption, an effect that could further impair mucociliary clearance in CF airways.
Conclusions
Our results suggest that rescue of F508del-CFTR by low temperature cannot be easily mimicked by small molecules and that compounds with closer transcriptional and functional effects need to be found.
Elsevier