Negative regulation of osteoclastogenesis by ectodomain shedding of receptor activator of NF-κB ligand
A Hikita, I Yana, H Wakeyama, M Nakamura… - Journal of Biological …, 2006 - jbc.org
Receptor activator of NF-κB ligand (RANKL) is a transmembrane glycoprotein that has an
essential role in the development of osteoclasts. The extracellular portion of RANKL is
cleaved proteolytically to produce soluble RANKL, but definite RANKL sheddase (s) and the
physiologic function of RANKL shedding have not yet been determined. In the present study,
we found that matrix metalloproteinase (MMP) 14 and a disintegrin and metalloproteinase
(ADAM) 10 have strong RANKL shedding activity. In Western blot analysis, soluble RANKL …
essential role in the development of osteoclasts. The extracellular portion of RANKL is
cleaved proteolytically to produce soluble RANKL, but definite RANKL sheddase (s) and the
physiologic function of RANKL shedding have not yet been determined. In the present study,
we found that matrix metalloproteinase (MMP) 14 and a disintegrin and metalloproteinase
(ADAM) 10 have strong RANKL shedding activity. In Western blot analysis, soluble RANKL …