[HTML][HTML] Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis

J Wu, Z Huang, J Ren, Z Zhang, P He, Y Li, J Ma… - Cell research, 2013 - nature.com
J Wu, Z Huang, J Ren, Z Zhang, P He, Y Li, J Ma, W Chen, Y Zhang, X Zhou, Z Yang, SQ Wu…
Cell research, 2013nature.com
Mixed lineage kinase domain-like protein (Mlkl) was recently found to interact with receptor
interacting protein 3 (Rip3) and to be essential for tumor necrosis factor (TNF)-induced
programmed necrosis (necroptosis) in cultured cell lines. We have generated Mlkl-deficient
mice by transcription activator-like effector nucleases (TALENs)-mediated gene disruption
and found Mlkl to be dispensable for normal mouse development as well as immune cell
development. Mlkl-deficient mouse embryonic fibroblasts (MEFs) and macrophages both …
Abstract
Mixed lineage kinase domain-like protein (Mlkl) was recently found to interact with receptor interacting protein 3 (Rip3) and to be essential for tumor necrosis factor (TNF)-induced programmed necrosis (necroptosis) in cultured cell lines. We have generated Mlkl-deficient mice by transcription activator-like effector nucleases (TALENs)-mediated gene disruption and found Mlkl to be dispensable for normal mouse development as well as immune cell development. Mlkl-deficient mouse embryonic fibroblasts (MEFs) and macrophages both showed resistance to necrotic but not apoptotic stimuli. Mlkl-deficient MEFs and macrophages were indistinguishable from wild-type cells in their ability to activate NF-κB, ERK, JNK, and p38 in response to TNF and lipopolysaccharides (LPS), respectively. Consistently, Mlkl-deficient macrophages and mice exhibited normal interleukin-1β (IL-1β), IL-6, and TNF production after LPS treatment. Mlkl deficiency protects mice from cerulean-induced acute pancreatitis, a necrosis-related disease, but has no effect on polymicrobial septic shock-induced animal death. Our results provide genetic evidence for the role of Mlkl in necroptosis.
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