[HTML][HTML] Site-specific phosphorylation of the p65 protein subunit mediates selective gene expression by differential NF-κB and RNA polymerase II promoter recruitment

K Hochrainer, G Racchumi, J Anrather - Journal of Biological Chemistry, 2013 - ASBMB
Phosphorylation of NF-κB plays an important role in modulating transcriptional activity of NF-
κB independently of inhibitor of κB (IκB) proteins. For the p65 subunit, multiple
phosphorylation sites have been mapped in and adjacent to both the N-terminal Rel
homology domain and the C-terminal transactivation domain. Their impact on NF-κB-
dependent transcription, however, has never been assessed at a broader level. In this study,
we evaluate the importance of differential p65 phosphorylation on four serine acceptor sites …