We describe a gradient elution reversed-phase high-performance liquid chromatographic
approach for isolation of individual glycerophospholipid molecular species which greatly
improves resolution and reduces run time compared to isocratic techniques. Separations
were optimized and elution order and retention time data established by synthesizing 37
different homogeneous phospholipids comprising the major alkylacyl, diacyl and
plasmalogen molecular species in samples derived from mammalian sources. Empirical …