[HTML][HTML] Structural analysis of Siah1-Siah-interacting protein interactions and insights into the assembly of an E3 ligase multiprotein complex
E Santelli, M Leone, C Li, T Fukushima… - Journal of Biological …, 2005 - ASBMB
Siah1 is the central component of a multiprotein E3 ubiquitin ligase complex that targets β-
catenin for destruction in response to p53 activation. The E3 complex comprises, in addition
to Siah1, Siah-interacting protein (SIP), the adaptor protein Skp1, and the F-box protein Ebi.
Here we show that SIP engages Siah1 by means of two elements, both of which are
required for mediating β-catenin destruction in cells. An N-terminal dimerization domain of
SIP sits across the saddle-shaped upper surface of Siah1, with two extended legs packing …
catenin for destruction in response to p53 activation. The E3 complex comprises, in addition
to Siah1, Siah-interacting protein (SIP), the adaptor protein Skp1, and the F-box protein Ebi.
Here we show that SIP engages Siah1 by means of two elements, both of which are
required for mediating β-catenin destruction in cells. An N-terminal dimerization domain of
SIP sits across the saddle-shaped upper surface of Siah1, with two extended legs packing …