Ubc9 and protein inhibitor of activated STAT 1 activate chicken ovalbumin upstream promoter-transcription factor I-mediated human CYP11B2 gene transcription
I Kurihara, H Shibata, S Kobayashi, N Suda… - Journal of Biological …, 2005 - ASBMB
Aldosterone synthase (CYP11B2) is involved in the final steps of aldosterone biosynthesis
and expressed exclusively in the adrenal zona glomerulosa cells. Using an electrophoretic
mobility shift assay, we demonstrate that COUP-TFI binds to the–129/–114 element (Ad5) of
human CYP11B2 promoter. Transient transfection in H295R adrenal cells demonstrated that
COUP-TFI enhanced CYP11B2 reporter activity. However, the reporter construct with
mutated Ad5 sequences showed reduced basal and COUP-TFI-enhanced activity …
and expressed exclusively in the adrenal zona glomerulosa cells. Using an electrophoretic
mobility shift assay, we demonstrate that COUP-TFI binds to the–129/–114 element (Ad5) of
human CYP11B2 promoter. Transient transfection in H295R adrenal cells demonstrated that
COUP-TFI enhanced CYP11B2 reporter activity. However, the reporter construct with
mutated Ad5 sequences showed reduced basal and COUP-TFI-enhanced activity …
