A mutation in sigma‐1 receptor causes juvenile amyotrophic lateral sclerosis

A Al‐Saif, F Al‐Mohanna, S Bohlega - Annals of neurology, 2011 - Wiley Online Library
A Al‐Saif, F Al‐Mohanna, S Bohlega
Annals of neurology, 2011Wiley Online Library
Objective: Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder
characterized by loss of motor neurons in the brain and spinal cord, leading to muscle
weakness and eventually death from respiratory failure. ALS is familial in about 10% of
cases, with SOD1 mutations accounting for 20% of familial cases. Here we describe a
consanguineous family segregating juvenile ALS in an autosomal recessive pattern and
describe the genetic variant responsible for the disorder. Methods: We performed …
Objective
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by loss of motor neurons in the brain and spinal cord, leading to muscle weakness and eventually death from respiratory failure. ALS is familial in about 10% of cases, with SOD1 mutations accounting for 20% of familial cases. Here we describe a consanguineous family segregating juvenile ALS in an autosomal recessive pattern and describe the genetic variant responsible for the disorder.
Methods
We performed homozygosity mapping and direct sequencing to detect the genetic variant and tested the effect of this variant on a motor neuron‐like cell line model (NSC34) expressing the wild‐type or mutant gene.
Results
We identified a shared homozygosity region in affected individuals that spans ∼120kbp on chromosome 9p13.3 containing 9 RefSeq genes. Sequencing the SIGMAR1 gene revealed a mutation affecting a highly conserved amino acid located in the transmembrane domain of the encoded protein, sigma‐1 receptor. The mutated protein showed an aberrant subcellular distribution in NSC34 cells. Furthermore, cells expressing the mutant protein were less resistant to apoptosis induced by endoplasmic reticulum stress.
Interpretation
Sigma‐1 receptors are known to have neuroprotective properties, and recently Sigmar1 knockout mice have been described to have motor deficiency. Our findings emphasize the role of sigma‐1 receptors in motor neuron function and disease. ANN NEUROL 2011;
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