[HTML][HTML] PTMScan direct: identification and quantification of peptides from critical signaling proteins by immunoaffinity enrichment coupled with LC-MS/MS
MP Stokes, CL Farnsworth, A Moritz, JC Silva… - Molecular & Cellular …, 2012 - ASBMB
Proteomic studies of post-translational modifications by metal affinity or antibody-based
methods often employ data-dependent analysis, providing rich data sets that consist of
randomly sampled identified peptides because of the dynamic response of the mass
spectrometer. This can complicate the primary goal of programs for drug development,
mutational analysis, and kinase profiling studies, which is to monitor how multiple nodes of
known, critical signaling pathways are affected by a variety of treatment conditions. Cell …
methods often employ data-dependent analysis, providing rich data sets that consist of
randomly sampled identified peptides because of the dynamic response of the mass
spectrometer. This can complicate the primary goal of programs for drug development,
mutational analysis, and kinase profiling studies, which is to monitor how multiple nodes of
known, critical signaling pathways are affected by a variety of treatment conditions. Cell …