Direct Activation of Protein Kinase C by 1α, 25-Dihydroxyvitamin D3 (∗)
SJ Slater, MB Kelly, FJ Taddeo, JD Larkin… - Journal of Biological …, 1995 - jbc.org
The key metabolite of vitamin D 3, 1α, 25-dihydroxyvitamin D 3 (1, 25-D 3), induces rapid
cellular responses that constitute a so-called" non-genomic" response. This effect is
distinguished from its" classic" genomic role in calcium homeostasis involving the nuclear 1,
25-D 3 receptor. Evidence is presented that protein kinase C (PKC) is directly activated by 1,
25-D 3 at physiological concentrations (EC 50= 16±1 nM). The effect was demonstrable with
single PKC-α,-γ, and-∊ isoform preparations, assayed in a system containing only purified …
cellular responses that constitute a so-called" non-genomic" response. This effect is
distinguished from its" classic" genomic role in calcium homeostasis involving the nuclear 1,
25-D 3 receptor. Evidence is presented that protein kinase C (PKC) is directly activated by 1,
25-D 3 at physiological concentrations (EC 50= 16±1 nM). The effect was demonstrable with
single PKC-α,-γ, and-∊ isoform preparations, assayed in a system containing only purified …
