Nasosorption as a minimally invasive sampling procedure: mucosal viral load and inflammation in primary RSV bronchiolitis
RS Thwaites, K Ito, JMS Chingono… - The Journal of …, 2017 - academic.oup.com
RS Thwaites, K Ito, JMS Chingono, M Coates, HC Jarvis, T Tunstall, L Anderson-Dring…
The Journal of infectious diseases, 2017•academic.oup.comBackground. Existing respiratory mucosal sampling methods are flawed, particularly in a
pediatric bronchiolitis setting. Methods. Twenty-four infants with bronchiolitis were recruited:
12 were respiratory syncytial virus (RSV)–positive, 12 were RSV-negative. Infants were
sampled by nasosorption and nasopharyngeal aspiration (NPA). Results. Nasosorption was
well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not
NPA) correlated with length of hospital stay (P=. 04) and requirement for mechanical …
pediatric bronchiolitis setting. Methods. Twenty-four infants with bronchiolitis were recruited:
12 were respiratory syncytial virus (RSV)–positive, 12 were RSV-negative. Infants were
sampled by nasosorption and nasopharyngeal aspiration (NPA). Results. Nasosorption was
well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not
NPA) correlated with length of hospital stay (P=. 04) and requirement for mechanical …
Background
Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting.
Methods
Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)–positive, 12 were RSV-negative. Infants were sampled by nasosorption and nasopharyngeal aspiration (NPA).
Results
Nasosorption was well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not NPA) correlated with length of hospital stay (P = .04) and requirement for mechanical ventilation (P = .03). Nasosorption (but not NPA) levels of interferon γ, interleukin 1β, CCL5/RANTES, and interleukin 10 (IL-10) were elevated in RSV+ bronchiolitis (all P < .05), furthermore CCL5 and IL-10 correlated with RSV load (P < .05).
Conclusions
Nasosorption allowed measurement of RSV load and the mucosal inflammatory response in infants.
Oxford University Press