Publisher Summary DT-diaphorase is a highly active diaphorase in the soluble fraction of rat
liver homogenates, which catalyzes the oxidation of nicotine adenine dinucleotide, reduced
nicotine adenine dinucleotide hydrogenase (NADH), and nicotinamide adenine dinucleotide
phosphate (NADPH) at equal rates. This chapter discusses the purification, properties, and
function of DT-diaphorase. Most of the present knowledge about DT-diaphorase has been
obtained in studies of the cytosolic enzyme, which was first purified from both rat and beef …