Whole-animal functional and developmental imaging with isotropic spatial resolution

RK Chhetri, F Amat, Y Wan, B Höckendorf… - Nature …, 2015 - nature.com
Nature methods, 2015nature.com
Imaging fast cellular dynamics across large specimens requires high resolution in all
dimensions, high imaging speeds, good physical coverage and low photo-damage. To meet
these requirements, we developed isotropic multiview (IsoView) light-sheet microscopy,
which rapidly images large specimens via simultaneous light-sheet illumination and
fluorescence detection along four orthogonal directions. Combining these four views by
means of high-throughput multiview deconvolution yields images with high resolution in all …
Abstract
Imaging fast cellular dynamics across large specimens requires high resolution in all dimensions, high imaging speeds, good physical coverage and low photo-damage. To meet these requirements, we developed isotropic multiview (IsoView) light-sheet microscopy, which rapidly images large specimens via simultaneous light-sheet illumination and fluorescence detection along four orthogonal directions. Combining these four views by means of high-throughput multiview deconvolution yields images with high resolution in all three dimensions. We demonstrate whole-animal functional imaging of Drosophila larvae at a spatial resolution of 1.1-2.5 μm and temporal resolution of 2 Hz for several hours. We also present spatially isotropic whole-brain functional imaging in Danio rerio larvae and spatially isotropic multicolor imaging of fast cellular dynamics across gastrulating Drosophila embryos. Compared with conventional light-sheet microscopy, IsoView microscopy improves spatial resolution at least sevenfold and decreases resolution anisotropy at least threefold. Compared with existing high-resolution light-sheet techniques, IsoView microscopy effectively doubles the penetration depth and provides subsecond temporal resolution for specimens 400-fold larger than could previously be imaged.
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