Characteristics of an established human glioma cell line, KNS-42
I TAKESHITA, T TAKAKI, M KURAMITSU… - Neurologia medico …, 1987 - jstage.jst.go.jp
I TAKESHITA, T TAKAKI, M KURAMITSU, S NAGASAKA, T MACHI, H OGAWA, H EGAMI…
Neurologia medico-chirurgica, 1987•jstage.jst.go.jpWe established a human glioma cell line derived from a malignant glioma and evaluated it
by im munochemical techniques and antibodies to astroglial (glial fibrillary acidic protein:
GFAP, S-100 protein), oligodendroglial (myelin basic protein, galactocerebroside), neuronal
(neuron-specific eno lase: NSE, neurofilament triplet proteins), and mesenchymal (vimentin,
fibronectin) antigens. The cell line was epithelial in sparse culture and glial in dense culture.
GFAP was expressed by most cells in sparse culture and primarily by overlying cells in …
by im munochemical techniques and antibodies to astroglial (glial fibrillary acidic protein:
GFAP, S-100 protein), oligodendroglial (myelin basic protein, galactocerebroside), neuronal
(neuron-specific eno lase: NSE, neurofilament triplet proteins), and mesenchymal (vimentin,
fibronectin) antigens. The cell line was epithelial in sparse culture and glial in dense culture.
GFAP was expressed by most cells in sparse culture and primarily by overlying cells in …
Abstract
We established a human glioma cell line derived from a malignant glioma and evaluated it by im munochemical techniques and antibodies to astroglial (glial fibrillary acidic protein: GFAP, S-100 protein), oligodendroglial (myelin basic protein, galactocerebroside), neuronal (neuron-specific eno lase: NSE, neurofilament triplet proteins), and mesenchymal (vimentin, fibronectin) antigens. The cell line was epithelial in sparse culture and glial in dense culture. GFAP was expressed by most cells in sparse culture and primarily by overlying cells in dense culture. The amount of GFAP depended on the cell density and ranged from 530 to 990 ng/mg of protein. The cells contained much more vimentin than GFAP. Neurofilament proteins, S-100 protein, myelin basic protein, and galactocerebroside were undetectable. The cells synthesized a small amount of fibronectin and released it into the medium. The amount of NSE was unrelated to cell density, ranged from 13 to 19 ng/mg of protein, and was present in greater quantity in cells cultured in a medium containing 1 ul/ml of sodium lactate. We conclude that KNS-42 is a permanent astrocytic glioma cell line; it expresses GFAP, NSE, vimen tin, and fibronectin, and is morphologically and functionally dependent on its environment.
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