Lipopolysaccharide of Pantoea agglomerans 7460: O-specific polysaccharide and lipid A structures and biological activity
EL Zdorovenko, AA Kadykova, AS Shashkov… - Carbohydrate …, 2020 - Elsevier
Lipopolysaccharide (LPS) was isolated from Pantoea agglomerans 7460 cells by phenol-
water extraction. Mild acid degradation allowed to separate OPS and lipid A. Lipid A was
analyzed by negative-ion mode ESI MS and found to consist mainly of hexaacylated
derivative containing biphosphorylated GlcN disaccharide, four 14: 0 (3-OH), 18: 0 and 12: 0
fatty acids. The structure of the O-specific polysaccharide was established by chemical, NMR
and computational methods: Image 2 The LPS of Р. agglomerans 7460 showed low level of …
water extraction. Mild acid degradation allowed to separate OPS and lipid A. Lipid A was
analyzed by negative-ion mode ESI MS and found to consist mainly of hexaacylated
derivative containing biphosphorylated GlcN disaccharide, four 14: 0 (3-OH), 18: 0 and 12: 0
fatty acids. The structure of the O-specific polysaccharide was established by chemical, NMR
and computational methods: Image 2 The LPS of Р. agglomerans 7460 showed low level of …