Inhibition of osteoclast bone resorption by disrupting vacuolar H+-ATPase a3-B2 subunit interaction
N Kartner, Y Yao, K Li, GJ Crasto, A Datti… - Journal of Biological …, 2010 - jbc.org
Vacuolar H+-ATPases (V-ATPases) are highly expressed in ruffled borders of bone-
resorbing osteoclasts, where they play a crucial role in skeletal remodeling. To discover
protein-protein interactions with the a subunit in mammalian V-ATPases, a GAL4 activation
domain fusion library was constructed from an in vitro osteoclast model, receptor activator of
NF-κB ligand-differentiated RAW 264.7 cells. This library was screened with a bait construct
consisting of a GAL4 binding domain fused to the N-terminal domain of V-ATPase a3 …
resorbing osteoclasts, where they play a crucial role in skeletal remodeling. To discover
protein-protein interactions with the a subunit in mammalian V-ATPases, a GAL4 activation
domain fusion library was constructed from an in vitro osteoclast model, receptor activator of
NF-κB ligand-differentiated RAW 264.7 cells. This library was screened with a bait construct
consisting of a GAL4 binding domain fused to the N-terminal domain of V-ATPase a3 …